Quantification of silver nanoparticle uptake and distribution within individual human macrophages by FIB/SEM slice and view

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dc.contributor.authorGührs, Erik
dc.contributor.authorSchneider, Michael
dc.contributor.authorGünther, Christian M.
dc.contributor.authorHessing, Piet
dc.contributor.authorHeitz, Karen
dc.contributor.authorWittke, Doreen
dc.contributor.authorOliver, Ana López-Serrano
dc.contributor.authorJakubowski, Norbert
dc.contributor.authorPlendl, Johanna
dc.contributor.authorEisebitt, Stefan
dc.contributor.authorHaase, Andrea
dc.date.accessioned2018-12-03T13:10:37Z
dc.date.available2018-12-03T13:10:37Z
dc.date.issued2017-03-21
dc.description.abstractBackground Quantification of nanoparticle (NP) uptake in cells or tissues is very important for safety assessment. Often, electron microscopy based approaches are used for this purpose, which allow imaging at very high resolution. However, precise quantification of NP numbers in cells and tissues remains challenging. The aim of this study was to present a novel approach, that combines precise quantification of NPs in individual cells together with high resolution imaging of their intracellular distribution based on focused ion beam/ scanning electron microscopy (FIB/SEM) slice and view approaches. Results We quantified cellular uptake of 75 nm diameter citrate stabilized silver NPs (Ag 75 Cit) into an individual human macrophage derived from monocytic THP-1 cells using a FIB/SEM slice and view approach. Cells were treated with 10 μg/ml for 24 h. We investigated a single cell and found in total 3138 ± 722 silver NPs inside this cell. Most of the silver NPs were located in large agglomerates, only a few were found in clusters of fewer than five NPs. Furthermore, we cross-checked our results by using inductively coupled plasma mass spectrometry and could confirm the FIB/SEM results. Conclusions Our approach based on FIB/SEM slice and view is currently the only one that allows the quantification of the absolute dose of silver NPs in individual cells and at the same time to assess their intracellular distribution at high resolution. We therefore propose to use FIB/SEM slice and view to systematically analyse the cellular uptake of various NPs as a function of size, concentration and incubation time.en
dc.description.sponsorshipTU Berlin, Open-Access-Mittel - 2017de
dc.identifier.issn1477-3155
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/8644
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-7778
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc610 Medizin und Gesundheitde
dc.subject.othernanoparticlesen
dc.subject.otherFIB/SEM slice and viewen
dc.subject.otherabsolute doseen
dc.subject.othercellular internalizationen
dc.subject.othermacrophageen
dc.titleQuantification of silver nanoparticle uptake and distribution within individual human macrophages by FIB/SEM slice and viewen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber21en
dcterms.bibliographicCitation.doi10.1186/s12951-017-0255-8en
dcterms.bibliographicCitation.journaltitleJournal of Nanobiotechnologyen
dcterms.bibliographicCitation.originalpublishernameBioMed Centralen
dcterms.bibliographicCitation.originalpublisherplaceLondonen
dcterms.bibliographicCitation.volume15en
tub.accessrights.dnbfreeen
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Optik und Atomare Physik::FG Röntgenoptik und Nanometer-Optikde
tub.affiliation.facultyFak. 2 Mathematik und Naturwissenschaftende
tub.affiliation.groupFG Röntgenoptik und Nanometer-Optikde
tub.affiliation.instituteInst. Optik und Atomare Physikde
tub.publisher.universityorinstitutionTechnische Universität Berlinen

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