An optimized protocol for assessment of sputum macrorheology in health and muco-obstructive lung disease

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dc.contributor.authorVöller, Mirjam
dc.contributor.authorAddante, Annalisa
dc.contributor.authorRulff, Hanna
dc.contributor.authorvon Lospichl, Benjamin
dc.contributor.authorGräber, Simon Y.
dc.contributor.authorDuerr, Julia
dc.contributor.authorLauster, Daniel
dc.contributor.authorHaag, Rainer
dc.contributor.authorGradzielski, Michael
dc.contributor.authorMall, Marcus A.
dc.date.accessioned2022-10-05T12:54:22Z
dc.date.available2022-10-05T12:54:22Z
dc.date.issued2022-08-05
dc.date.updated2022-08-19T05:44:14Z
dc.description.abstractBackground: Airway mucus provides important protective functions in health and abnormal viscoelasticity is a hallmark of muco-obstructive lung diseases such as cystic fibrosis (CF). However, previous studies of sputum macrorheology from healthy individuals and patients with CF using different experimental protocols yielded in part discrepant results and data on a systematic assessment across measurement settings and conditions remain limited. Objectives: The aim of this study was to develop an optimized and reliable protocol for standardized macrorheological measurements of airway mucus model systems and native human sputum from healthy individuals and patients with muco-obstructive lung disease. Methods: Oscillatory rheological shear measurements were performed using bovine submaxillary mucin (BSM) at different concentrations (2% and 10% solids) and sputum samples from healthy controls (n = 10) and patients with CF (n = 10). Viscoelastic properties were determined by amplitude and frequency sweeps at 25°C and 37°C with or without solvent trap using a cone-plate geometry. Results: Under saturated atmosphere, we did not observe any temperature-dependent differences in 2% and 10% BSM macrorheology, whereas in the absence of evaporation control 10% BSM demonstrated a significantly higher viscoelasticity at 37°C. Similarly, during the measurements without evaporation control at 37°C we observed a substantial increase in the storage modulus G′ and the loss modulus G″ of the highly viscoelastic CF sputum but not in the healthy sputum. Conclusion: Our data show systematically higher viscoelasticity of CF compared to healthy sputum at 25°C and 37°C. For measurements at the higher temperature using a solvent trap to prevent evaporation is essential for macrorheological analysis of mucus model systems and native human sputum. Another interesting finding is that the viscoelastic properties are not much sensitive to the applied experimental deformation and yield robust results despite their delicate consistency. The optimized protocol resulting from this work will facilitate standardized quantitative assessment of abnormalities in viscoelastic properties of airway mucus and response to muco-active therapies in patients with CF and other muco-obstructive lung diseases.en
dc.description.sponsorshipDFG, 431232613, SFB 1449: Dynamische Hydrogele an Biogrenzflächenen
dc.description.sponsorshipDFG, 414044773, Open Access Publizieren 2021 - 2022 / Technische Universität Berlinen
dc.identifier.eissn1664-042X
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/17550
dc.identifier.urihttps://doi.org/10.14279/depositonce-16331
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc610 Medizin und Gesundheitde
dc.subject.othermucus
dc.subject.othersputum
dc.subject.otherbovine submaxillary mucin
dc.subject.othermacrorheology
dc.subject.othersolvent trap
dc.subject.othercystic fibrosis
dc.subject.othermuco-obstructive lung disease
dc.titleAn optimized protocol for assessment of sputum macrorheology in health and muco-obstructive lung disease
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.articlenumber912049
dcterms.bibliographicCitation.doi10.3389/fphys.2022.912049
dcterms.bibliographicCitation.journaltitleFrontiers in Physiology
dcterms.bibliographicCitation.originalpublishernameFrontiers
dcterms.bibliographicCitation.originalpublisherplaceLausanne
dcterms.bibliographicCitation.volume13
tub.accessrights.dnbfree
tub.affiliationFak. 2 Mathematik und Naturwissenschaften::Inst. Chemie::FG Physikalische Chemie / Molekulare Materialwissenschaften
tub.publisher.universityorinstitutionTechnische Universität Berlin

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