Bacterial Growth in Chloride and Perchlorate Brines: Halotolerances and Salt Stress Responses of Planococcus halocryophilus

dc.contributor.authorHeinz, Jacob
dc.contributor.authorWaajen, Annemiek C.
dc.contributor.authorAiro, Alessandro
dc.contributor.authorAlibrandi, Armando
dc.contributor.authorSchirmack, Janosch
dc.contributor.authorSchulze-Makuch, Dirk
dc.date.accessioned2019-08-28T13:32:39Z
dc.date.available2019-08-28T13:32:39Z
dc.date.issued2019-08-06
dc.description.abstractExtraterrestrial environments encompass physicochemical conditions and habitats that are unknown on Earth, such as perchlorate-rich brines that can be at least temporarily stable on the martian surface. To better understand the potential for life in these cold briny environments, we determined the maximum salt concentrations suitable for growth (MSCg) of six different chloride and perchlorate salts at 25°C and 4°C for the extremotolerant cold- and salt-adapted bacterial strain Planococcus halocryophilus. Growth was measured through colony-forming unit (CFU) counts, while cellular and colonial phenotypic stress responses were observed through visible light, fluorescence, and scanning electron microscopy. Our data show the following: (1) The tolerance to high salt concentrations can be increased through a stepwise inoculation toward higher concentrations. (2) Ion-specific factors are more relevant for the growth limitation of P. halocryophilus in saline solutions than single physicochemical parameters like ionic strength or water activity. (3) P. halocryophilus shows the highest microbial sodium perchlorate tolerance described so far. However, (4) MSCg values are higher for all chlorides compared to perchlorates. (5) The MSCg for calcium chloride was increased by lowering the temperature from 25°C to 4°C, while sodium- and magnesium-containing salts can be tolerated at 25°C to higher concentrations than at 4°C. (6) Depending on salt type and concentration, P. halocryophilus cells show distinct phenotypic stress responses such as novel types of colony morphology on agar plates and biofilm-like cell clustering, encrustation, and development of intercellular nanofilaments. This study, taken in context with previous work on the survival of extremophiles in Mars-like environments, suggests that high-concentrated perchlorate brines on Mars might not be habitable to any present organism on Earth, but extremophilic microorganisms might be able to evolve thriving in such environments.en
dc.description.sponsorshipEC/FP7/339231/EU/Habitability of Martian Environments: Exploring the Physiological and Environmental Limits of Life/HOMEen
dc.identifier.eissn1557-8070
dc.identifier.issn1531-1074
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/9905
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-8917
dc.language.isoenen
dc.relation.ispartof10.14279/depositonce-8847
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en
dc.subject.ddc520 Astronomie und zugeordnete Wissenschaftende
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherbrinesen
dc.subject.othersalten
dc.subject.othergrowthen
dc.subject.otherMarsen
dc.subject.otherperchlorateen
dc.subject.otherhalotoleranceen
dc.titleBacterial Growth in Chloride and Perchlorate Brines: Halotolerances and Salt Stress Responses of Planococcus halocryophilusen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1089/ast.2019.2069en
dcterms.bibliographicCitation.issue2en
dcterms.bibliographicCitation.journaltitleAstrobiologyen
dcterms.bibliographicCitation.originalpublishernameMary Ann Lieberten
dcterms.bibliographicCitation.originalpublisherplaceNew Yorken
dcterms.bibliographicCitation.volume20en
tub.accessrights.dnbfreeen
tub.affiliationFak. 2 Mathematik und Naturwissenschaften>Zentrum für Astronomie und Astrophysikde
tub.affiliation.facultyFak. 2 Mathematik und Naturwissenschaftende
tub.affiliation.instituteZentrum für Astronomie und Astrophysikde
tub.publisher.universityorinstitutionTechnische Universität Berlinen
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