Exploring the suitability of ecosystem metabolomes to assess imprints of brownification and nutrient enrichment on lakes

dc.contributor.authorFonvielle, Jeremy A.
dc.contributor.authorGiling, Darren P.
dc.contributor.authorDittmar, Thorsten
dc.contributor.authorBerger, Stella A.
dc.contributor.authorNejstgaard, Jens C.
dc.contributor.authorSolheim, Anne Lyche
dc.contributor.authorGessner, Mark O.
dc.contributor.authorGrossart, Hans-Peter
dc.contributor.authorSinger, Gabriel
dc.date.accessioned2021-08-03T09:45:10Z
dc.date.available2021-08-03T09:45:10Z
dc.date.issued2021-05-12
dc.description.abstractLoadings of colored dissolved organic matter (cDOM) and nutrients affect lake ecosystem functioning in opposite ways, rendering assessments of combined effects challenging. We used the “ecosystem metabolome” as a conceptual framework to overcome this problem by characterizing the chemically diverse pool of DOM in lakes. The underlying rationale is that the diversity of dissolved metabolites bears the legacy of allochthonous inputs, autochthonous primary production, and a wealth of organic matter transformations resulting from microbial or photodegradation. Our objective was to assess whether high-resolution mass-spectrometric analyses can unlock that information on DOM origin and transformation pathways as well as environmental drivers imprinting the lake ecosystem metabolome. We performed a large-scale enclosure experiment to assess the influences of brownification and nutrient enrichment on the composition and diversity of DOM, and a complementary bottle incubation to isolate the effect of photodegradation. For validation, we assessed whether the same patterns emerge from published observational data from 109 Swedish lakes. Ultra-high-resolution mass spectrometry distinguished ∼3000 metabolites in solid-phase extracts of lake water. Network analysis revealed five metabolite clusters that could be related to different source processes based on molecular weight, position in van Krevelen diagrams and assignment to molecular categories (peptides, lipids, etc.). Emergent DOM properties such as molecular diversity provided insights into the processes generating each of the five DOM clusters. Overall, our data suggest that the thousands of molecular formulas comprising ecosystem metabolomes of lakes arise from few major processes and reflect imprints of environmental drivers such as brownification and nutrient enrichment.en
dc.description.sponsorshipEC/FP7/603378/EU/Managing Aquatic ecosystems and water Resources under multiple Stress/MARSen
dc.description.sponsorshipBMBF, 01LC1501G, BIBS-Verbund: Bridging in Biodiversity Science (BIBS) - Teilprojekt 7: LakeScapeLabs, Kopplung in Metaökosystemenen
dc.description.sponsorshipBMBF, 033L041B, Verbundprojekt: NITROLIMIT - Stickstofflimitation in Binnengewässern - ist Stickstoffreduktion ökologisch sinnvoll und wirtschaftlich vertretbar? Teilprojekt: Verfügbarkeit von gelösten organischen N-Verbindungen, Produktion von Lachgas und Stickstoffeinträgeen
dc.identifier.eissn2169-8961
dc.identifier.issn0148-0227
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/13474
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-12260
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc577 Ökologiede
dc.subject.otherdissolved organic matteren
dc.subject.otherDOMen
dc.subject.otherfluorescenceen
dc.subject.otherhigh-resolution mass spectrometryen
dc.subject.othermetabolomicsen
dc.titleExploring the suitability of ecosystem metabolomes to assess imprints of brownification and nutrient enrichment on lakesen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumbere2020JG005903en
dcterms.bibliographicCitation.doi10.1029/2020JG005903en
dcterms.bibliographicCitation.issue5en
dcterms.bibliographicCitation.journaltitleJournal of Geophysical Researchen
dcterms.bibliographicCitation.originalpublishernameWileyen
dcterms.bibliographicCitation.originalpublisherplaceNew York, NYen
dcterms.bibliographicCitation.volume126en
tub.accessrights.dnbfreeen
tub.affiliationFak. 6 Planen Bauen Umwelt::Inst. Ökologie::FG Angewandte Gewässerökologiede
tub.affiliation.facultyFak. 6 Planen Bauen Umweltde
tub.affiliation.groupFG Angewandte Gewässerökologiede
tub.affiliation.instituteInst. Ökologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen

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