Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142

dc.contributor.authorPolerecky, Lubos
dc.contributor.authorMasuda, Takako
dc.contributor.authorEichner, Meri
dc.contributor.authorRabouille, Sophie
dc.contributor.authorVancová, Marie
dc.contributor.authorKienhuis, Michiel V. M.
dc.contributor.authorBernát, Gabor
dc.contributor.authorBonomi-Barufi, Jose
dc.contributor.authorCampbell, Douglas Andrew
dc.contributor.authorClaquin, Pascal
dc.contributor.authorČervený, Jan
dc.contributor.authorGiordano, Mario
dc.contributor.authorKotabová, Eva
dc.contributor.authorKromkamp, Jacco
dc.contributor.authorLombardi, Ana Teresa
dc.contributor.authorLukeš, Martin
dc.contributor.authorPrášil, Ondrej
dc.contributor.authorStephan, Susanne
dc.contributor.authorSuggett, David
dc.contributor.authorZavřel, Tomas
dc.contributor.authorHalsey, Kimberly H.
dc.date.accessioned2021-02-25T09:37:01Z
dc.date.available2021-02-25T09:37:01Z
dc.date.issued2021-02-04
dc.date.updated2021-02-18T09:05:42Z
dc.description.abstractUnicellular nitrogen fixing cyanobacteria (UCYN) are abundant members of phytoplankton communities in a wide range of marine environments, including those with rapidly changing nitrogen (N) concentrations. We hypothesized that differences in N availability (N2 vs. combined N) would cause UCYN to shift strategies of intracellular N and C allocation. We used transmission electron microscopy and nanoscale secondary ion mass spectrometry imaging to track assimilation and intracellular allocation of 13C-labeled CO2 and 15N-labeled N2 or NO3 at different periods across a diel cycle in Cyanothece sp. ATCC 51142. We present new ideas on interpreting these imaging data, including the influences of pre-incubation cellular C and N contents and turnover rates of inclusion bodies. Within cultures growing diazotrophically, distinct subpopulations were detected that fixed N2 at night or in the morning. Additional significant within-population heterogeneity was likely caused by differences in the relative amounts of N assimilated into cyanophycin from sources external and internal to the cells. Whether growing on N2 or NO3, cells prioritized cyanophycin synthesis when N assimilation rates were highest. N assimilation in cells growing on NO3 switched from cyanophycin synthesis to protein synthesis, suggesting that once a cyanophycin quota is met, it is bypassed in favor of protein synthesis. Growth on NO3 also revealed that at night, there is a very low level of CO2 assimilation into polysaccharides simultaneous with their catabolism for protein synthesis. This study revealed multiple, detailed mechanisms underlying C and N management in Cyanothece that facilitate its success in dynamic aquatic environments.en
dc.identifier.eissn1664-302X
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/12674
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-11472
dc.language.isoenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherCrocosphaera subtropicaen
dc.subject.otherCyanotheceen
dc.subject.otherphotosynthesisen
dc.subject.othercarbon fixationen
dc.subject.othernitrogen fixationen
dc.subject.othernanoSIMSen
dc.subject.otherTEMen
dc.titleTemporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142en
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.articlenumber620915en
dcterms.bibliographicCitation.doi10.3389/fmicb.2021.620915en
dcterms.bibliographicCitation.journaltitleFrontiers in Microbiologyen
dcterms.bibliographicCitation.originalpublishernameFrontiersen
dcterms.bibliographicCitation.originalpublisherplaceLausanneen
dcterms.bibliographicCitation.volume12en
tub.accessrights.dnbfreeen
tub.affiliationFak. 6 Planen Bauen Umwelt>Inst. Ökologie>FG Angewandte Gewässerökologiede
tub.affiliation.facultyFak. 6 Planen Bauen Umweltde
tub.affiliation.groupFG Angewandte Gewässerökologiede
tub.affiliation.instituteInst. Ökologiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen
Files
Original bundle
Now showing 1 - 8 of 8
Loading…
Thumbnail Image
Name:
fmicb-12-620915.pdf
Size:
7.19 MB
Format:
Adobe Portable Document Format
Description:
Loading…
Thumbnail Image
Name:
Data_Sheet_1.pdf
Size:
3.06 MB
Format:
Adobe Portable Document Format
Description:
Loading…
Thumbnail Image
Name:
Data_Sheet_2.XLSX
Size:
78.12 KB
Format:
Microsoft Excel XML
Description:
Loading…
Thumbnail Image
Name:
fmicb-12-620915-g001.tif
Size:
4.84 MB
Format:
Tag Image File Format
Description:
Loading…
Thumbnail Image
Name:
fmicb-12-620915-g002.tif
Size:
2.62 MB
Format:
Tag Image File Format
Description:
Loading…
Thumbnail Image
Name:
fmicb-12-620915-g003.tif
Size:
4.88 MB
Format:
Tag Image File Format
Description:
Loading…
Thumbnail Image
Name:
fmicb-12-620915-g004.tif
Size:
4.89 MB
Format:
Tag Image File Format
Description:
Loading…
Thumbnail Image
Name:
fmicb-12-620915-g005.tif
Size:
1.03 MB
Format:
Tag Image File Format
Description:
Collections