The Fatty Acid Synthase of the Basidiomycete Omphalotus olearius Is a Single Polypeptide
| dc.contributor.author | Antelo, Luis | |
| dc.contributor.author | Schlipp, Angela | |
| dc.contributor.author | Hof, Carolin | |
| dc.contributor.author | Eisfeld, Katrin | |
| dc.contributor.author | Berg, Holger | |
| dc.contributor.author | Hornbogen, Till | |
| dc.contributor.author | Zocher, Rainer | |
| dc.contributor.author | Anke, Heidrun | |
| dc.date.accessioned | 2018-10-01T08:02:35Z | |
| dc.date.available | 2018-10-01T08:02:35Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Fatty acids are essential components of almost all biological membranes. Additionally, they are important in energy storage, as second messengers during signal transduction, and in post-translational protein modification. De novo synthesis of fatty acids is essential for almost all organisms, and entails the iterative elongation of the growing fatty acid chain through a set of reactions conserved in all kingdoms. During our work on the biosynthesis of secondary metabolites, a 450-kDa protein was detected by SDS-PAGE of enriched fractions from mycelial lysates from the basidiomycete Omphalotus olearius. Protein sequencing of this protein band revealed the presence of peptides with homology to both α and β subunits of the ascomycete fatty acid synthase (FAS) family. The FAS encoding gene of O. olearius was sequenced. The positions of its predicted 21 introns were verified. The gene encodes a 3931 amino acids single protein, with an equivalent of the ascomycetous β subunit at the N-terminus and the α subunit at the C-terminus. This is the first report on an FAS protein from a homobasidiomycete and also the first fungal FAS which is comprised of a single polypeptide. | en |
| dc.identifier.eissn | 1865-7125 | |
| dc.identifier.issn | 0939-5075 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/8236 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-7387 | |
| dc.language.iso | en | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ | |
| dc.subject.ddc | 570 Biowissenschaften; Biologie | de |
| dc.subject.other | Omphalotus olearius | en |
| dc.subject.other | fatty acid synthase | en |
| dc.subject.other | Basidiomycete | en |
| dc.title | The Fatty Acid Synthase of the Basidiomycete Omphalotus olearius Is a Single Polypeptide | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.doi | 10.1515/znc-2009-3-416 | |
| dcterms.bibliographicCitation.issue | 3-4 | |
| dcterms.bibliographicCitation.journaltitle | Zeitschrift für Naturforschung C | en |
| dcterms.bibliographicCitation.originalpublishername | De Gruyter | en |
| dcterms.bibliographicCitation.originalpublisherplace | Berlin | en |
| dcterms.bibliographicCitation.pageend | 250 | |
| dcterms.bibliographicCitation.pagestart | 244 | |
| dcterms.bibliographicCitation.volume | 64 | |
| tub.accessrights.dnb | free | |
| tub.affiliation | Fak. 2 Mathematik und Naturwissenschaften::Inst. Chemie | de |
| tub.affiliation.faculty | Fak. 2 Mathematik und Naturwissenschaften | de |
| tub.affiliation.institute | Inst. Chemie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | de |
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