Dielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?

dc.contributor.authorHenriksson, Anders
dc.contributor.authorNeubauer, Peter
dc.contributor.authorBirkholz, Mario
dc.date.accessioned2022-12-28T08:30:18Z
dc.date.available2022-12-28T08:30:18Z
dc.date.issued2022-09-23
dc.date.updated2022-11-10T12:56:07Z
dc.description.abstractThe performance of receptor-based biosensors is often limited by either diffusion of the analyte causing unreasonable long assay times or a lack of specificity limiting the sensitivity due to the noise of nonspecific binding. Alternating current (AC) electrokinetics and its effect on biosensing is an increasing field of research dedicated to address this issue and can improve mass transfer of the analyte by electrothermal effects, electroosmosis, or dielectrophoresis (DEP). Accordingly, several works have shown improved sensitivity and lowered assay times by order of magnitude thanks to the improved mass transfer with these techniques. To realize high sensitivity in real samples with realistic sample matrix avoiding nonspecific binding is critical and the improved mass transfer should ideally be specific to the target analyte. In this paper we cover recent approaches to combine biosensors with DEP, which is the AC kinetic approach with the highest selectivity. We conclude that while associated with many challenges, for several applications the approach could be beneficial, especially if more work is dedicated to minimizing nonspecific bindings, for which DEP offers interesting perspectives.
dc.description.sponsorshipBMBF, 13N15712, Evaneszente optische Felder zur ultrasensitiven Detektion von Pathogenen mittels integrierter Mikroring-Resonatoren und Dielektrophorese
dc.description.sponsorshipDFG, 414044773, Open Access Publizieren 2021 - 2022 / Technische Universität Berlinen
dc.identifier.eissn2079-6374
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/17887
dc.identifier.urihttps://doi.org/10.14279/depositonce-16676
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc570 Biowissenschaften; Biologiede
dc.subject.otherdielectrophoresis
dc.subject.otherbiosensor
dc.subject.otherdiffusion
dc.subject.othermass-transfer
dc.subject.othernon-specific interaction
dc.subject.otherAC electrokinetics
dc.titleDielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.articlenumber784
dcterms.bibliographicCitation.doi10.3390/bios12100784
dcterms.bibliographicCitation.issue10
dcterms.bibliographicCitation.journaltitleBiosensors
dcterms.bibliographicCitation.originalpublishernameMDPI
dcterms.bibliographicCitation.originalpublisherplaceBasel
dcterms.bibliographicCitation.volume12
dcterms.rightsHolder.referenceCreative-Commons-Lizenz
tub.accessrights.dnbfree
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioverfahrenstechnik
tub.publisher.universityorinstitutionTechnische Universität Berlin

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