BPA Endocrine Disruptor Detection at the Cutting Edge: FPIA and ELISA Immunoassays

dc.contributor.authorRaysyan, Anna
dc.contributor.authorZwigart, Sandro D.
dc.contributor.authorEremin, Sergei A.
dc.contributor.authorSchneider, Rudolf J.
dc.date.accessioned2023-09-08T15:18:17Z
dc.date.available2023-09-08T15:18:17Z
dc.date.issued2023-06-19
dc.date.updated2023-07-19T09:02:37Z
dc.description.abstractBPA is a chemical commonly used in the production of polymer-based materials that can have detrimental effects on the thyroid gland and impact human reproductive health. Various expensive methods, such as liquid and gas chromatography, have been suggested for detecting BPA. The fluorescence polarization immunoassay (FPIA) is an inexpensive and efficient homogeneous mix-and-read method that allows for high-throughput screening. FPIA offers high specificity and sensitivity and can be carried out in a single phase within a timeframe of 20–30 min. In this study, new tracer molecules were designed that linked the fluorescein fluorophore with and without a spacer to the bisphenol A moiety. To assess the influence of the C6 spacer on the sensitivity of an assay based on the respective antibody, hapten–protein conjugates were synthesized and assessed for performance in an ELISA setup, and this resulted in a highly sensitive assay with a detection limit of 0.05 g/L. The lowest limit of detection was reached by employing the spacer derivate in the FPIA and was 1.0 μg/L, working range from 2 to 155 μg/L. The validation of the methods was conducted using actual samples compared to LC–MS/MS, which served as the reference method. The FPIA and ELISA both demonstrated satisfactory concordance.en
dc.identifier.eissn2079-6374
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/19606
dc.identifier.urihttps://doi.org/10.14279/depositonce-18405
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc600 Technik, Medizin, angewandte Wissenschaften::660 Chemische Verfahrenstechnik::660 Chemische Verfahrenstechnik
dc.subject.otherFPIA
dc.subject.otherELISA
dc.subject.otherimmunoassay
dc.subject.otherbisphenol A
dc.subject.otherendocrine disruptor
dc.titleBPA Endocrine Disruptor Detection at the Cutting Edge: FPIA and ELISA Immunoassays
dc.typeArticle
dc.type.versionpublishedVersion
dcterms.bibliographicCitation.articlenumber664
dcterms.bibliographicCitation.doi10.3390/bios13060664
dcterms.bibliographicCitation.issue6
dcterms.bibliographicCitation.journaltitleBiosensors
dcterms.bibliographicCitation.originalpublishernameMDPI
dcterms.bibliographicCitation.originalpublisherplaceBasel
dcterms.bibliographicCitation.volume13
dcterms.rightsHolder.referenceCreative-Commons-Lizenz
tub.accessrights.dnbfree
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioanalytik
tub.publisher.universityorinstitutionTechnische Universität Berlin

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