High-cell-density fed-batch cultivations of Vibrio natriegens
| dc.contributor.author | Thiele, Isabel | |
| dc.contributor.author | Gutschmann, Björn | |
| dc.contributor.author | Aulich, Linus | |
| dc.contributor.author | Girard, Marcel | |
| dc.contributor.author | Neubauer, Peter | |
| dc.contributor.author | Riedel, Sebastian L. | |
| dc.date.accessioned | 2021-12-06T18:44:29Z | |
| dc.date.available | 2021-12-06T18:44:29Z | |
| dc.date.issued | 2021-05-19 | |
| dc.description.abstract | Objectives: With generation times of less than 10 min under optimal conditions, the halophilic Vibrio natriegens is the fastest growing non-pathogenic bacterium isolated so far. The availability of the full genome and genetic engineering tools and its ability to utilize a wide range of carbon sources make V. natriegens an attractive host for biotechnological production processes. However, high-cell-density cultivations, which are desired at industrial-scale have not been described so far. Results: In this study we report fed-batch cultivations of V. natriegens in deep-well plates and lab-scale bioreactor cultivations at different temperatures in mineral salt medium (MSM). Upon switching from exponential glucose to constant glucose-feeding cell death was induced. Initial NaCl concentrations of 15–18 g L −1 and a temperature reduction from 37 to 30 °C had a positive effect on cell growth. The maximal growth rate in MSM with glucose was 1.36 h −1 with a specific oxygen uptake rate of 22 mmol g CDW −1 h −1 . High biomass yields of up to 55 g L −1 after only 12 h were reached. Conclusions: The shown fed-batch strategies demonstrate the potential of V. natriegens as a strong producer in industrial biotechnology. | en |
| dc.description.sponsorship | TU Berlin, Open-Access-Mittel – 2021 | en |
| dc.identifier.eissn | 1573-6776 | |
| dc.identifier.issn | 0141-5492 | |
| dc.identifier.pmid | 34009528 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/13988 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-12761 | |
| dc.language.iso | en | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject.ddc | 660 Chemische Verfahrenstechnik | de |
| dc.subject.other | vibrio natriegens | en |
| dc.subject.other | deep-well plate | en |
| dc.subject.other | fast growing microorganism | en |
| dc.subject.other | fed-batch | en |
| dc.subject.other | high-cell-density cultivations | en |
| dc.subject.other | parallel bioreactor cultivations | en |
| dc.title | High-cell-density fed-batch cultivations of Vibrio natriegens | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.doi | 10.1007/s10529-021-03147-5 | en |
| dcterms.bibliographicCitation.issue | 9 | en |
| dcterms.bibliographicCitation.journaltitle | Biotechnology Letters | en |
| dcterms.bibliographicCitation.originalpublishername | Springer Nature | en |
| dcterms.bibliographicCitation.originalpublisherplace | Dordrecht [u.a.] | en |
| dcterms.bibliographicCitation.pageend | 1733 | en |
| dcterms.bibliographicCitation.pagestart | 1723 | en |
| dcterms.bibliographicCitation.volume | 43 | en |
| tub.accessrights.dnb | free | en |
| tub.affiliation | Fak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioverfahrenstechnik | de |
| tub.affiliation.faculty | Fak. 3 Prozesswissenschaften | de |
| tub.affiliation.group | FG Bioverfahrenstechnik | de |
| tub.affiliation.institute | Inst. Biotechnologie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | en |
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