Interaction of proteins and amino acids with iso-α-acids during wort preparation in the brewhouse

dc.contributor.authorGänz, Nele
dc.contributor.authorBecher, Tobias
dc.contributor.authorDrusch, Stephan
dc.contributor.authorTitze, Jean
dc.date.accessioned2022-02-18T09:26:14Z
dc.date.available2022-02-18T09:26:14Z
dc.date.issued2021-12-02
dc.description.abstractThis paper investigates the binding behavior of iso-α-acids from hops on free wort amino acids and proteins concerning the wort production process in breweries. The studies were carried out with different amino acids, bovine serum albumin and wort. To identify the nature of reaction between iso-α-acids and these substances, analyses of free amino nitrogen, HPLC and isothermal titration calorimetry were performed. According to the results, the iso-α-acids do not form covalent bonds with free amino acids of wort. However, iso-α-acids, especially isohumulone and isoadhumulone, form ionic bonds with wort proteins. A distinction must be made between proteins that are present in the hot trub, and those that are still dissolved in the hot wort. Proteins that are already coagulated and precipitated no longer react with iso-α-acids. Future experiments will investigate whether the established ionic bonds between iso-α-acids and proteins from the wort preparation process are maintained during fermentation until the finished beer or beer foam. If this is the case, which is induced by the experiments, there is a measurable loss of iso-α-acids in the hot wort, but at the same time, a gain for the later beer foam retention, as the iso-α-acids will stabilize it.en
dc.description.sponsorshipTU Berlin, Open-Access-Mittel – 2022en
dc.identifier.eissn1438-2385
dc.identifier.issn1438-2377
dc.identifier.urihttps://depositonce.tu-berlin.de/handle/11303/16443
dc.identifier.urihttp://dx.doi.org/10.14279/depositonce-15219
dc.language.isoenen
dc.relation.ispartof10.14279/depositonce-12784
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subject.ddc633 Feld- und Plantagenfrüchtede
dc.subject.ddc664 Lebensmitteltechnologiede
dc.subject.otheriso-α-acidsen
dc.subject.otheramino acidsen
dc.subject.otherproteinsen
dc.subject.otherbeer productionen
dc.subject.otherITCen
dc.subject.otherHPLCen
dc.titleInteraction of proteins and amino acids with iso-α-acids during wort preparation in the brewhouseen
dc.typeArticleen
dc.type.versionpublishedVersionen
dcterms.bibliographicCitation.doi10.1007/s00217-021-03926-4en
dcterms.bibliographicCitation.journaltitleEuropean Food Research and Technologyen
dcterms.bibliographicCitation.originalpublishernameSpringer Natureen
dcterms.bibliographicCitation.originalpublisherplaceHeidelbergen
dcterms.bibliographicCitation.pageend750en
dcterms.bibliographicCitation.pagestart741en
dcterms.bibliographicCitation.volume248en
tub.accessrights.dnbfreeen
tub.affiliationFak. 3 Prozesswissenschaften::Inst. Lebensmitteltechnologie und Lebensmittelchemie::FG Lebensmitteltechnologie und -materialwissenschaftende
tub.affiliation.facultyFak. 3 Prozesswissenschaftende
tub.affiliation.groupFG Lebensmitteltechnologie und -materialwissenschaftende
tub.affiliation.instituteInst. Lebensmitteltechnologie und Lebensmittelchemiede
tub.publisher.universityorinstitutionTechnische Universität Berlinen

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