Savas, SumeyraErsoy, AylinGulmez, YakupKilic, SelcukLevent, BelkisAltintas, Zeynep2019-09-112019-09-112018-08-27https://depositonce.tu-berlin.de/handle/11303/10001http://dx.doi.org/10.14279/depositonce-8992Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL−1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection.en540 Chemie und zugeordnete WissenschaftenSalmonella spp.pathogen detectionantibody biosensorDNA biosensormicrofluidic-based electrochemical sensornanoparticle enhanced bio-detectioninfectious diseasesArticle2019-08-011996-1944