Surface enhanced vibrational spectroscopic evidence for an alternative DNA-independent redox activation of endonuclease III

Moe, ElinSezer, MuratHildebrandt, PeterTodorovic, Smilja2017-10-252017-10-2520151359-7345https://depositonce.tu-berlin.de/handle/11303/6975http://dx.doi.org/10.14279/depositonce-6314Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.Surface enhanced vibrational spectro-electrochemistry of endonuclease III provides direct evidence that the [4Fe-4S] cluster is responsible for the enzyme redox activity, and that this process is not exclusively DNA-mediated, as currently proposed. We report the first surface enhanced resonance Raman spectrum of a [4Fe-4S](2+) cluster containing enzyme.en540 Chemie und zugeordnete WissenschaftenSurface enhanced vibrational spectroscopic evidence for an alternative DNA-independent redox activation of endonuclease IIIArticle1364-548X25608720