Characterization of the [3Fe-4S](0/1+) cluster from the D14C variant of Pyrococcus furiosus ferredoxin via combined NRVS and DFT analyses

Lauterbach, LarsGee, Leland B.Pelmenschikov, VladimirJenney, Francis E., Jr.Kamali, SaeedYoda, YoshitakaAdams, Michael W. W.Cramer, Stephen P.2017-10-242017-10-2420161477-9226https://depositonce.tu-berlin.de/handle/11303/6905http://dx.doi.org/10.14279/depositonce-6244Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.The D14C variant of Pyrococcus furiosus ferredoxin provides an extraordinary framework to investigate a [3Fe-4S] cluster at two oxidation levels and compare the results to its physiologic [4Fe-4S] counterpart in the very same protein. Our spectroscopic and computational study reveals vibrational property changes related to the electronic and structural aspects of both Fe-S clusters.en540 Chemie und zugeordnete WissenschaftenCharacterization of the [3Fe-4S](0/1+) cluster from the D14C variant of Pyrococcus furiosus ferredoxin via combined NRVS and DFT analysesArticle1477-923427063792