H-2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O-2-tolerant hydrogenase and a P450 monooxygenase

Lonsdale, Thomas H.Lauterbach, LarsMalca, Sumire HondaNestl, Bettina M.Hauer, BernhardLenz, Oliver2017-10-252017-10-2520151359-7345https://depositonce.tu-berlin.de/handle/11303/6933http://dx.doi.org/10.14279/depositonce-6272An in vivo biotransformation system is presented that affords the hydroxylation of n-octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD(+)-reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H-2-driven NADH cofactor regeneration even in the presence of O-2, the co-substrate of monooxygenase.en540 Chemie und zugeordnete WissenschaftenH-2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O-2-tolerant hydrogenase and a P450 monooxygenaseArticle1364-548X26394141