dc.contributor.author	Schultz, Fabien
dc.contributor.author	Osuji, Ogechi Favour
dc.contributor.author	Nguyen, Anh
dc.contributor.author	Anywar, Godwin
dc.contributor.author	Scheel, John R.
dc.contributor.author	Caljon, Guy
dc.contributor.author	Pieters, Luc
dc.contributor.author	Garbe, Leif-Alexander
dc.date.accessioned	2021-07-26T11:22:21Z
dc.date.available	2021-07-26T11:22:21Z
dc.date.issued	2021-06-30
dc.date.updated	2021-07-14T08:57:45Z
dc.description.abstract	We investigated the potential antimalarial and toxicological effects of 16 medicinal plants frequently used by traditional healers to treat malaria, fever, and related disorders in the Greater Mpigi region in Uganda. Species studied were Albizia coriaria, Cassine buchananii, Combretum molle, Erythrina abyssinica, Ficus saussureana, Harungana madagascariensis, Leucas calostachys, Microgramma lycopodioides, Morella kandtiana, Plectranthus hadiensis, Securidaca longipedunculata, Sesamum calycinum subsp. angustifolium, Solanum aculeastrum, Toddalia asiatica, Warburgia ugandensis, and Zanthoxylum chalybeum. In addition, the traditional healers indicated that P. hadiensis is used as a ritual plant to boost fertility and prepare young women and teenagers for motherhood in some Ugandan communities where a high incidence of rapidly growing large breast masses in young female patients was observed (not necessarily breast cancer). We present results from various in vitro experiments performed with 56 different plant extracts, namely, 1) an initial assessment of the 16 species regarding their traditional use in the treatment of malaria by identifying promising plant extract candidates using a heme biocrystallization inhibition library screen; 2) follow-up investigations of antiprotozoal effects of the most bioactive crude extracts against chloroquine-resistant P. falciparum K1; 3) a cytotoxicity counterscreen against human MRC-5SV2 lung fibroblasts; 4) a genotoxicity evaluation of the extract library without and with metabolic bioactivation with human S9 liver fraction; and 5) an assessment of the mutagenicity of the ritual plant P. hadiensis. A total of seven extracts from five plant species were selected for antiplasmodial follow-up investigations based on their hemozoin formation inhibition activity in the heme biocrystallization assay. Among other extracts, an ethyl acetate extract of L. calostachys leaves exhibited antiplasmodial activity against P. falciparum K1 (IC50 value: 5.7 µg/ml), which was further characterized with a selectivity index of 2.6 (CC50 value: 14.7 µg/ml). The experiments for assessment of potential procarcinogenic properties of plant extracts via evaluation of in vitro mutagenicity and genotoxicity indicated that few extracts cause mutations. The species T. asiatica showed the most significant genotoxic effects on both bacterial test strains (without metabolic bioactivation at a concentration of 500 µg/plate). However, none of the mutagenic extracts from the experiments without metabolic bioactivation retained their genotoxic activity after metabolic bioactivation of the plant extract library through pre-incubation with human S9 liver fraction. While this study did not show that P. hadiensis has genotoxic properties, it did provide early stage support for the therapeutic use of the medicinal plants from the Greater Mpigi region.	en
dc.description.sponsorship	BMBF, 13FH026IX5, IngenieurNachwuchs 2015: Schutz der Gesundheit durch Einsatz biologischer Fungizide in der Landwirtschaft - Anwendung von Trihydroxy-octadecensäuren (TriOH) als natürliche Pflanzenschutzmittel zur Sicherung einer gesunden Ernährung (OxiLiFungi)	en
dc.description.sponsorship	BMBF, 13FH066PX5, FHprofUnt 2015: Modifikation von Proteinen bei der technischen Prozessierung von Melasse und Vinasse: Einfluss auf die Produktion von Bioethanol und Biogas	en
dc.description.sponsorship	DFG, 414051096, Open Access Publizieren 2020 - 2021 / Hochschule Neubrandenburg	en
dc.identifier.eissn	1663-9812
dc.identifier.uri	https://depositonce.tu-berlin.de/handle/11303/13447
dc.identifier.uri	http://dx.doi.org/10.14279/depositonce-12233
dc.language.iso	en	en
dc.relation.ispartof	10.14279/depositonce-12048	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject.ddc	610 Medizin und Gesundheit	de
dc.subject.other	malaria	en
dc.subject.other	antiprotozoal	en
dc.subject.other	cytotoxicity	en
dc.subject.other	genotoxicity	en
dc.subject.other	medicinal plants	en
dc.subject.other	Uganda	en
dc.subject.other	Mpigi	en
dc.subject.other	ethnopharmacology	en
dc.title	Pharmacological Assessment of the Antiprotozoal Activity, Cytotoxicity and Genotoxicity of Medicinal Plants Used in the Treatment of Malaria in the Greater Mpigi Region in Uganda	en
dc.type	Article	en
dc.type.version	publishedVersion	en
dcterms.bibliographicCitation.articlenumber	678535	en
dcterms.bibliographicCitation.doi	10.3389/fphar.2021.678535	en
dcterms.bibliographicCitation.journaltitle	Frontiers in Pharmacology	en
dcterms.bibliographicCitation.originalpublishername	Frontiers	en
dcterms.bibliographicCitation.originalpublisherplace	Lausanne	en
dcterms.bibliographicCitation.volume	12	en
tub.accessrights.dnb	free	en
tub.affiliation	Fak. 3 Prozesswissenschaften::Inst. Biotechnologie::FG Bioanalytik	de
tub.affiliation.faculty	Fak. 3 Prozesswissenschaften	de
tub.affiliation.group	FG Bioanalytik	de
tub.affiliation.institute	Inst. Biotechnologie	de
tub.publisher.universityorinstitution	Technische Universität Berlin	en

Pharmacological Assessment of the Antiprotozoal Activity, Cytotoxicity and Genotoxicity of Medicinal Plants Used in the Treatment of Malaria in the Greater Mpigi Region in Uganda

Files

Original bundle

License bundle

Collections