Studies on Herbicide Binding in Photosystem II Membrane Fragments from Spinach
| dc.contributor.author | Fromme, R. | |
| dc.contributor.author | Renger, G. | |
| dc.date.accessioned | 2018-11-10T16:39:00Z | |
| dc.date.available | 2018-11-10T16:39:00Z | |
| dc.date.issued | 1990 | |
| dc.description.abstract | The mechanism of atrazine binding and its modification by Chelex-100-induced Ca2+ depletion and proteolytic degradation by trypsin, was analyzed in PS II membrane fragments from spinach. It was found: 1) Chelex-100 treatment leads in a comparatively slow process (t1/2 = 5 - 10 min) to Ca2+ re moval from a site that is characterized by a high affinity as reflected by KD values of the order of 10-7M. The number of these binding sites was found to be almost one per PS II in samples washed twice with Ca2+ -free buffer. 2) Chelex-100 treatment does not affect the affinity of atrazine binding but increases the susceptibility to proteolytic attack by trypsin. 3) The electron transport activity is only slightly affected by Chelex-100 treatment. 4) The atrazine binding exhibits a rather small T-dependence within the physiological range of 7 °C to 27 °C. The implications of these findings for herbicide binding are discussed. | en |
| dc.identifier.eissn | 1865-7125 | |
| dc.identifier.issn | 0939-5075 | |
| dc.identifier.uri | https://depositonce.tu-berlin.de/handle/11303/8501 | |
| dc.identifier.uri | http://dx.doi.org/10.14279/depositonce-7647 | |
| dc.language.iso | en | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ | |
| dc.subject.ddc | 570 Biowissenschaften; Biologie | de |
| dc.subject.other | atrazine binding | en |
| dc.subject.other | Photosystem II | en |
| dc.subject.other | Ca2+ effects | en |
| dc.subject.other | temperature dependence | en |
| dc.subject.other | mild proteolysis | en |
| dc.title | Studies on Herbicide Binding in Photosystem II Membrane Fragments from Spinach | en |
| dc.type | Article | en |
| dc.type.version | publishedVersion | en |
| dcterms.bibliographicCitation.doi | 10.1515/znc-1990-0511 | |
| dcterms.bibliographicCitation.issue | 5 | |
| dcterms.bibliographicCitation.journaltitle | Zeitschrift für Naturforschung C | de |
| dcterms.bibliographicCitation.originalpublishername | De Gruyter | en |
| dcterms.bibliographicCitation.originalpublisherplace | Berlin | en |
| dcterms.bibliographicCitation.pageend | 378 | |
| dcterms.bibliographicCitation.pagestart | 373 | |
| dcterms.bibliographicCitation.volume | 45 | |
| tub.accessrights.dnb | free | |
| tub.affiliation | Fak. 2 Mathematik und Naturwissenschaften::Inst. Chemie | de |
| tub.affiliation.faculty | Fak. 2 Mathematik und Naturwissenschaften | de |
| tub.affiliation.institute | Inst. Chemie | de |
| tub.publisher.universityorinstitution | Technische Universität Berlin | de |
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